![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K1445001:Design
Designed by: Josephina Hendrix Group: iGEM14_CU-Boulder (2014-09-25)
Endogenous Type II CRISPR-Cas9 phagemid
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 2179
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 4458
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 5400
Illegal BsaI.rc site found at 5377
Design Notes
We have experienced some toxic effects by the pCas9 part so we suggest growing part-containing cells in lower concentrations of antibiotic, even if plasmid contains a high copy origin of replication.
Source
The M13 origin of replication from Litmus28i and part BBa_K1218011 from the registry.
References
Jiang, Wenyan, et al. 2013. RNA-guided editing of bacterial genomes using CRISPR-Cas systems. Nat Biotechnol.